Muscle weakness is a common symptom of both long-time alcoholics and patients with mitochondrial disease. Now researchers have found a common link: mitochondria that are unable to self-repair. The results will be published online April 21 in The Journal of Cell Biology. The link to self-repair provides researchers both a new way to diagnose mitochondrial disease, and a new drug target.
Mitochondria - organelles that produce the energy needed for muscle, brain, and every other cell in the body - repair their broken components by fusing with other mitochondria and exchanging their contents. Damaged parts are segregated for recycling and replaced with properly functioning proteins donated from healthy mitochondria.
While fusion is one major method for mitochondrial quality control in many types of cells, researchers have puzzled over the repair mechanism in skeletal muscle - a type of tissue that relies constantly on mitochondria for power, making repair a frequent necessity. However, mitochondria are squeezed so tightly in between the packed fibers of muscle cells, that most researchers assumed that fusion among mitochondria in this tissue type was impossible.
An inkling that fusion might be important for the normal muscle function came from research on two mitochondrial diseases: Autosomal Dominant Optical Atropy (ADOA) disease, and a type of Charcot-Marie-Tooth disease (CMT). A symptom of both disease is muscle weakness and patients with both these diseases carry a mutation in one of the three genes involved in mitochondrial fusion.
To investigate whether mitochondria in the muscle could indeed fuse to regenerate, first author Veronica Eisner, Ph.D., a postdoctoral fellow at Thomas Jefferson University created a system to tag the mitochondria in skeletal muscle of rats with two different colors and then watch if they mingled. First, she created a rat model whose mitochondria expressed the color red at all times. She also genetically engineered the mitochondria in the cells to turn green when zapped with a laser, creating squares of green-shining mitochondria within the red background. To her surprise, the green mitochondria not only mingled with the red, exchanging contents, but were also able to travel to other areas where only red-colored mitochondria had been. The results were exciting in that they showed “for the first time that mitochondrial fusion occurs in muscle cells,” says Dr. Eisner.
The researcher team, led by Dr. Gyorgy Hajnoczky, M.D., Ph.D., Director of Jefferson’s MitoCare Center and professor in the department of Pathology, Anatomy & Cell Biology, then showed that of the mitofusin (Mfn) fusion proteins, Mfn1 was most important in skeletal muscle cells.
Once they had identified Mfn1, they were able to test whether mitochondrial fusion was the culprit in other examples of muscle weakness, such as alcoholism. One long-term symptom of alcoholism is the loss of muscle strength. The researchers showed that the Mfn1 abundance went down as much as 50 percent in rats on a regular alcohol diet-while other fusion proteins were unchanged, and that this decrease was coupled with a massive decrease in mitochondrial fusion. When Mfn1 was restored, so was the mitochondrial fusion. They also linked the decreased Mfn1 and mitochondrial fusion to increased muscle fatigue.
“That alcohol can have a specific effect on this one gene involved in mitochondrial fusion suggests that other environmental factors may also specifically alter mitochondrial fusion and repair,” says Dr. Hajnoczky.
“The work provides more evidence to support the concept that fission and fusion - or mitochondrial dynamics - may be responsible for more than just a subset of mitochondrial diseases we know of,” says Dr. Hajnoczky. “In addition, knowing the proteins involved in the process gives us the possibility of developing a drug.”
This work was supported by PEW Latin American Fellows Program in the Biomedical Sciences and the Duchenne Parent Project, Netherlands fellowships to Veronika Eisner and by a grant from NIAAA (AA017773) to Gyorgy Hajnoczky.
The authors declare that they have no conflict of interest.
Thomas Jefferson University (TJU), the largest freestanding academic medical center in Philadelphia, is nationally renowned for medical and health sciences education and innovative research. Founded in 1824, TJU includes Jefferson Medical College (JMC), one of the largest private medical schools in the country and ranked among the nation’s best medical schools by U.S. News & World Report, and the Jefferson Schools of Nursing, Pharmacy, Health Professions, Population Health and the Graduate School of Biomedical Sciences. Jefferson University Physicians is TJU’s multi-specialty physician practice consisting of the full-time faculty of JMC. Thomas Jefferson University partners with its clinical affiliate, Thomas Jefferson University Hospitals.
Article reference: V. Eisner et al., “Mitochondrial fusion is frequent in skeletal muscle and supports excitation contraction coupling,” Journal of Cell Biology, DOI:10.1083/jcb.201312066, 2014
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