Coombs' test - direct
Key points
- The direct Coombs' test measures the presence of antibodies on the surface of red blood cells.
- Blood is drawn from a vein, usually from the inside of the elbow or the back of the hand.
- The area is cleansed with antiseptic and punctured with a sharp needle or a lancet.
- The preparation you can provide for this test depends on your child's age, previous experiences, and level of trust.
Definition
The direct Coombs' test measures the presence of antibodies on the surface of red blood cells.
How the test is performed
Blood is drawn from a vein, usually from the inside of the elbow or the back of the hand. The puncture site is cleaned with antiseptic. An elastic band is placed around the upper arm to apply pressure and cause the vein to swell with blood.
A needle is inserted into the vein, and the blood is collected in an air-tight vial or a syringe. During the procedure, the band is removed to restore circulation. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.
In infants or young children:
The area is cleansed with antiseptic and punctured with a sharp needle or a lancet. The blood may be collected in a pipette (small glass tube), on a slide, onto a test strip, or into a small container. A bandage may be applied to the puncture site if there is any bleeding.
How to prepare for the test
No special preparation is necessary for this test.
Infants and children:
The preparation you can provide for this test depends on your child's age, previous experiences, and level of trust. For general information regarding how you can prepare your child, see the following topics:
- Infant test or procedure preparation (birth to 1 year)
- Toddler test or procedure preparation (1 to 3 years)
- Preschooler test or procedure preparation (3 to 6 years)
- Schoolage test or procedure preparation (6 to 12 years)
- Adolescent test or procedure preparation (12 to 18 years)
How the test will feel
When the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing or a bruise may develop at the site where the needle was inserted.
Why the test is performed
The direct Coombs' test is used to detect autoantibodies against your own red blood cells (RBCs). Many diseases and drugs (quinidine, methyldopa, and procainamide) can lead to production of these antibodies.
These antibodies sometimes destroy red blood cells and cause anemia. This test is sometimes performed to diagnose the cause of anemia, jaundice, or abnormalities in the appearance of RBCs under the microscope.
Written and revised by
David Cohen, M.D.
Our doctors write and review articles for accuracy and keep them up to date.
health.am provides general information, not medical advice. Always consult a qualified health professional about your own health.